| Gene | Ccdc22 |
| Chromosome | X |
| Deletion Start | 7,605,290 |
| Deletion End | 7,594,013 |
| Deletion Size | 11,278 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,604,659 | 7,604,682 | TCCTATGTAGCTTTCCCAAACCTC |
| TUP | 7,604,633 | 7,604,656 | AGATCTAGGAGGCCTTGCCATGCA |
| TUR | 7,604,612 | 7,604,630 | GGGCACCAGGACATTGTTC |
| TDF | 7,594,142 | 7,594,162 | GACAGAGATGGGCAAGAAGAC |
| TDP | 7,594,113 | 7,594,141 | TTTGAGCAACCTGGAGAAGATCTGTGAGG |
| TDR | 7,594,073 | 7,594,090 | CCCAAGAGGCCAGCATTC |
PCR Primers
| Primer | Sequence |
| SU | CCCAGTCGTAGCAATTATGTC |
| SD | CACACCGACTCTCCAAGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17324A-D8 | VGB6 | C57BL/6NTac | |
| 17324A-H1 | VGB6 | C57BL/6NTac | |
| 17324A-H5 | VGB6 | C57BL/6NTac | |
| 17324A-H10 | VGB6 | C57BL/6NTac | |
