| Gene | Cdc26 |
| Chromosome | 4 |
| Deletion Start | 62,402,801 |
| Deletion End | 62,394,768 |
| Deletion Size | 8,034 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,402,205 | 62,402,224 | CACCTCTGGGTCTCACAATC |
| TUP | 62,402,176 | 62,402,202 | TCTGCCTCATCTTCCTCATCAATCCCA |
| TUR | 62,402,138 | 62,402,160 | AGCACCCTGTCCTAACTGGAATG |
| TDF | 62,395,466 | 62,395,484 | CCAGTCTTCCAGGCTGTGG |
| TDP | 62,395,420 | 62,395,447 | TCATGACGTTGACCACTGTTCTCACTGA |
| TDR | 62,395,343 | 62,395,360 | GCTACAAAGGCCCAGCAG |
PCR Primers
| Primer | Sequence |
| SU | GCTCCAGTAATAAATGGTTGC |
| SD | GCTTGAAGGATGGTCAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 142 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17327A-B10 | VGB6 | C57BL/6NTac | |
| 17327A-C10 | VGB6 | C57BL/6NTac | |
| 17327A-C12 | VGB6 | C57BL/6NTac | |
| 17327A-D1 | VGB6 | C57BL/6NTac | |
| 17327A-F10 | VGB6 | C57BL/6NTac | |
| 17327A-G1 | VGB6 | C57BL/6NTac | |
