| Gene | Cdc42ep2 |
| Chromosome | 19 |
| Deletion Start | 5,918,672 |
| Deletion End | 5,917,984 |
| Deletion Size | 689 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,918,619 | 5,918,638 | GCCGCAAAGGCAAGAAAGAG |
| TUP | 5,918,595 | 5,918,617 | AGCTTCGGGACCTGCTGTCCTCA |
| TUR | 5,918,540 | 5,918,561 | CGCTGCCAATGTGAATAGTGTG |
| TDF | 5,918,165 | 5,918,185 | ACAATGGGATGAGCCCTGAGC |
| TDP | 5,918,140 | 5,918,161 | AGGCCGAGGAGCCCTTCCTGTC |
| TDR | 5,918,105 | 5,918,123 | GGTCCACATGCAGGGACAG |
PCR Primers
| Primer | Sequence |
| SU | ATGGGTTCCTTCATTTCAGC |
| SD | TTATTGCGACCAGAATTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17328A-A3 | VGB6 | C57BL/6NTac | |
| 17328A-C1 | VGB6 | C57BL/6NTac | |
| 17328A-C6 | VGB6 | C57BL/6NTac | |
| 17328A-C7 | VGB6 | C57BL/6NTac | |
| 17328A-H3 | VGB6 | C57BL/6NTac | |
| 17328A-F3 | VGB6 | C57BL/6NTac | |
