| Gene | Cdkn2d |
| Chromosome | 9 |
| Deletion Start | 21,290,946 |
| Deletion End | 21,288,897 |
| Deletion Size | 2,050 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,290,757 | 21,290,777 | CCCATCCCTACAAGTCTCCTG |
| TUP | | | CCGGGTTGGTGATCCCT |
| TUR | 21,290,698 | 21,290,717 | CCACCCACCCAACATCAGAG |
| TDF | 21,289,578 | 21,289,600 | GAGAGAATTCCAGGACATCCAGA |
| TDP | 21,289,547 | 21,289,572 | ATCCTGTCTCCGACAAGGATGGGGGT |
| TDR | 21,289,521 | 21,289,542 | CCCCACACACACACCTTCTAAG |
PCR Primers
| Primer | Sequence |
| SU | GAAAGGAGGGAGGGTGAGTC |
| SD | GCTCACACTTCAGGGCTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17329A-A3 | VGB6 | C57BL/6NTac | |
| 17329A-H11 | VGB6 | C57BL/6NTac | |
| 17329A-C12 | VGB6 | C57BL/6NTac | |
| 17329A-D2 | VGB6 | C57BL/6NTac | |
| 17329A-D12 | VGB6 | C57BL/6NTac | |
