| Gene | Cinp |
| Chromosome | 12 |
| Deletion Start | 110,889,069 |
| Deletion End | 110,873,887 |
| Deletion Size | 15,183 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 110,888,864 | 110,888,884 | CGGTGATGTGCGTGAAGTGTC |
| TUP | 110,888,839 | 110,888,863 | AGTGGTGTGACGCTCAGAGCATCCC |
| TUR | 110,888,814 | 110,888,834 | GTCAGGCTTGGCTCCAATGTG |
| TDF | 110,874,187 | 110,874,205 | CCACTGCCAGCACTGATTC |
| TDP | 110,874,161 | 110,874,186 | TGGCTTGAGCTAAGGACAGTGCTTTC |
| TDR | 110,874,132 | 110,874,150 | GCAGCAGAACAGACACCAC |
PCR Primers
| Primer | Sequence |
| SU | CGCTCCCGAACTCACCTC |
| SD | TCCACAGTGCTCTCAACTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17330A-A2 | VGB6 | C57BL/6NTac | |
| 17330A-B9 | VGB6 | C57BL/6NTac | |
| 17330A-G8 | VGB6 | C57BL/6NTac | |
| 17330A-C11 | VGB6 | C57BL/6NTac | |
| 17330A-D2 | VGB6 | C57BL/6NTac | |
| 17330A-C1 | VGB6 | C57BL/6NTac | |
