| Gene | Clstn3 |
| Chromosome | 6 |
| Deletion Start | 124,464,481 |
| Deletion End | 124,431,514 |
| Deletion Size | 32,968 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,463,564 | 124,463,585 | GGGCAGCAGGTAGACATAAGAC |
| TUP | 124,463,526 | 124,463,554 | AAGGAGGGCTCATTTGGTAAGGGCATAAA |
| TUR | 124,463,501 | 124,463,519 | GAGCCAGGCACCCTACAAC |
| TDF | 124,432,113 | 124,432,134 | ATTGTGGTATGCGTGGGCTTTC |
| TDP | 124,432,087 | 124,432,112 | TGGTGCTTATGGTTATCCTCGGCCTC |
| TDR | 124,432,042 | 124,432,059 | TCCGCCAGTTCCTGAGAC |
PCR Primers
| Primer | Sequence |
| SU | TGACGTCATCCTGCAGTAGC |
| SD | CCCTCACGGACAGAACAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17331A-C9 | VGB6 | C57BL/6NTac | |
| 17331A-E2 | VGB6 | C57BL/6NTac | |
| 17331A-F11 | VGB6 | C57BL/6NTac | |
| 17331A-G8 | VGB6 | C57BL/6NTac | |
| 17331A-B1 | VGB6 | C57BL/6NTac | |
