| Gene | Col6a1 |
| Chromosome | 10 |
| Deletion Start | 76,725,910 |
| Deletion End | 76,709,521 |
| Deletion Size | 16,390 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,725,077 | 76,725,097 | CATGGCTCATGGGATCTTCTC |
| TUP | 76,725,056 | 76,725,076 | TGGCTCAGACTGCCCTGTGGA |
| TUR | 76,725,011 | 76,725,030 | AGCCTCAAGGCCACACTCTC |
| TDF | 76,709,622 | 76,709,643 | CCACCTATTCCGTGTACCAAAC |
| TDP | 76,709,601 | 76,709,621 | TACCAGGCCCTGCTACGTGGC |
| TDR | 76,709,559 | 76,709,577 | CTAGCCCAGTGCCACCTTC |
PCR Primers
| Primer | Sequence |
| SU | CAAAGGCGGTATTTGGAATG |
| SD | GCAGTGAGGAGGAAGACGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17332A-A4 | VGB6 | C57BL/6NTac | |
| 17332A-A12 | VGB6 | C57BL/6NTac | |
| 17332A-G11 | VGB6 | C57BL/6NTac | |
| 17332A-E2 | VGB6 | C57BL/6NTac | |
| 17332A-D2 | VGB6 | C57BL/6NTac | |
