| Gene | Cry2 |
| Chromosome | 2 |
| Deletion Start | 92,434,020 |
| Deletion End | 92,411,561 |
| Deletion Size | 22,460 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,433,847 | 92,433,865 | CATCCTCGACCCGTGGTTC |
| TUP | 92,433,824 | 92,433,845 | CGGCCTCCTCGTCTGTGGGCAT |
| TUR | 92,433,797 | 92,433,818 | ATAGGGTCCCCTCACCTCCATC |
| TDF | 92,411,918 | 92,411,940 | AGGGATCTGAGCACCAGAAGTTG |
| TDP | 92,411,898 | 92,411,916 | CCTTGCTGCCAGCAGGCCA |
| TDR | 92,411,846 | 92,411,863 | GCCTCCTTTGCAGCAGTG |
PCR Primers
| Primer | Sequence |
| SU | GTTAGAAAGGGCCCGGTAAC |
| SD | ACCCTCGCCAGAAATCAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17335B-B2 | VGB6 | C57BL/6NTac | |
| 17335B-C6 | VGB6 | C57BL/6NTac | |
| 17335B-C9 | VGB6 | C57BL/6NTac | |
| 17335B-G1 | VGB6 | C57BL/6NTac | |
| 17335C-C7 | VGB6 | C57BL/6NTac | |
