| Gene | Dgat1 |
| Chromosome | 15 |
| Deletion Start | 76,511,778 |
| Deletion End | 76,502,212 |
| Deletion Size | 9,567 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,511,009 | 76,511,029 | CAGGCTGCATGGCTTATTCAC |
| TUP | 76,510,981 | 76,511,004 | CTTCCAGCAAGTAGGAGGGCCTGA |
| TUR | 76,510,943 | 76,510,964 | AGCCCGAAGTCATTCTGGTAAC |
| TDF | 76,502,393 | 76,502,414 | CTGACTGTAGCCTCATGTGTTC |
| TDP | 76,502,370 | 76,502,392 | TCAGGTCCCACTGGCCTGGATTG |
| TDR | 76,502,335 | 76,502,355 | TGCATTGCCATAGTTCCCTTG |
PCR Primers
| Primer | Sequence |
| SU | CAGGAGGCTCCGCGCTCT |
| SD | AAACAGCAGCCATGTAACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17341B-F5 | VGB6 | C57BL/6NTac | |
| 17341B-G2 | VGB6 | C57BL/6NTac | |
| 17341B-G10 | VGB6 | C57BL/6NTac | |
| 17341B-H11 | VGB6 | C57BL/6NTac | |
