| Gene | Dgka |
| Chromosome | 10 |
| Deletion Start | 128,737,069 |
| Deletion End | 128,720,406 |
| Deletion Size | 16,664 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 128,737,049 | 128,737,069 | GCCAAAGAGAAGGGCCTCATC |
| TUP | 128,737,023 | 128,737,046 | CCCAGAAGACTTCGCTCAGCTGCA |
| TUR | 128,736,984 | 128,737,004 | TGGAAGGACCAGGAGAAAGAC |
| TDF | 128,720,989 | 128,721,007 | ACCAGGCTCAAGAGTGCTG |
| TDP | 128,720,967 | 128,720,987 | ACACCGGCTGGCCAAGTGCTC |
| TDR | 128,720,933 | 128,720,951 | TGGCTGGAGTCCTCCTTAC |
PCR Primers
| Primer | Sequence |
| SU | GGACCACAGAGCCTGAAGTG |
| SD | TTTCTGCAGTCTTCCCTTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17343A-C12 | VGB6 | C57BL/6NTac | |
| 17343A-D8 | VGB6 | C57BL/6NTac | |
| 17343A-D10 | VGB6 | C57BL/6NTac | |
| 17343A-F1 | VGB6 | C57BL/6NTac | |
| 17343A-F7 | VGB6 | C57BL/6NTac | |
