| Gene | Dhrs4 |
| Chromosome | 14 |
| Deletion Start | 55,490,005 |
| Deletion End | 55,478,906 |
| Deletion Size | 11,100 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,479,156 | 55,479,177 | GGACCTGAAATCGCTCCGAATC |
| TUP | 55,479,182 | 55,479,203 | ACCTGGCCCTGCCACAGGCTAG |
| TUR | 55,479,206 | 55,479,230 | AAGCCGAAGGCAACAGTGATACAAG |
| TDF | 55,489,656 | 55,489,676 | CCTGGGTCCTTTGGAAAGCAG |
| TDP | 55,489,677 | 55,489,702 | CTGGCCAATGCTCCTAACTGCTGAGC |
| TDR | 55,489,708 | 55,489,726 | TATCAGGTGGGAGCTGGTG |
PCR Primers
| Primer | Sequence |
| SU | AAGGTGGACCAGCAGAAGTG |
| SD | AGAGCAACGCGATCATCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17344A-A11 | VGB6 | C57BL/6NTac | |
| 17344A-D2 | VGB6 | C57BL/6NTac | |
| 17344A-D8 | VGB6 | C57BL/6NTac | |
| 17344A-H2 | VGB6 | C57BL/6NTac | |
| 17344A-H7 | VGB6 | C57BL/6NTac | |
| 17344A-G8 | VGB6 | C57BL/6NTac | |
