| Gene | Dhx32 |
| Chromosome | 7 |
| Deletion Start | 133,759,699 |
| Deletion End | 133,721,171 |
| Deletion Size | 38,529 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 133,759,670 | 133,759,691 | GCGGGAAGAAATGGATGAAGAG |
| TUP | 133,759,644 | 133,759,668 | AACTGGACCATCCGAACGCCTCTCC |
| TUR | 133,759,557 | 133,759,576 | GGGTTCAGCTCCAGATCCTC |
| TDF | 133,721,241 | 133,721,263 | CCAGATGCTACTGAGCAGAGATG |
| TDP | 133,721,209 | 133,721,235 | TCCAGTGACCTCTCCCAACATAGTGCA |
| TDR | 133,721,187 | 133,721,204 | GCTCCTGTTGCAGTGTCC |
PCR Primers
| Primer | Sequence |
| SU | TTGCTTTGAGCGTGTCTACG |
| SD | TCCAGACGACAGGATGAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 135 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17345A-B1 | VGB6 | C57BL/6NTac | |
| 17345A-G9 | VGB6 | C57BL/6NTac | |
