| Gene | Dnajb12 |
| Chromosome | 10 |
| Deletion Start | 59,896,505 |
| Deletion End | 59,879,681 |
| Deletion Size | 16,825 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,880,276 | 59,880,300 | CCTTAGCGAAATAAGACATTCCAAC |
| TUP | 59,880,301 | 59,880,325 | TCGCAGTCCTTCCTGCAGTCTCTTG |
| TUR | 59,880,332 | 59,880,353 | CAATCCATCCACTCCGAAACAC |
| TDF | 59,896,365 | 59,896,382 | ACCGAGCCCGCTACTTTG |
| TDP | 59,896,384 | 59,896,410 | TGACACAGACATGTACCACAGAGCACA |
| TDR | 59,896,432 | 59,896,451 | GCACCTCTGACAGCCGATTG |
PCR Primers
| Primer | Sequence |
| SU | CCTCCCTTCCATCCCTCTAC |
| SD | GCATCTTGCTCCATTTCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17346A-B4 | VGB6 | C57BL/6NTac | |
| 17346A-B10 | VGB6 | C57BL/6NTac | |
| 17346A-C9 | VGB6 | C57BL/6NTac | |
| 17346A-E6 | VGB6 | C57BL/6NTac | |
| 17346A-G8 | VGB6 | C57BL/6NTac | |
| 17346A-H9 | VGB6 | C57BL/6NTac | |
