| Gene | Dnajc12 |
| Chromosome | 10 |
| Deletion Start | 63,408,283 |
| Deletion End | 63,386,701 |
| Deletion Size | 21,583 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 63,386,726 | 63,386,745 | AGGGCAGCGAGGACTACTAC |
| TUP | 63,386,747 | 63,386,771 | CCTTGCTGGGATGCGATGAGCTCTC |
| TUR | 63,386,776 | 63,386,795 | GAACCTGCAACCCTGCTTAC |
| TDF | 63,408,078 | 63,408,097 | AGCCTCACTCCCACTGGTTG |
| TDP | 63,408,101 | 63,408,127 | TGGAGTCTTAACCGCAAGCTGTTTCAC |
| TDR | 63,408,165 | 63,408,186 | GGAGAAGAGGGAAGAGAAACAC |
PCR Primers
| Primer | Sequence |
| SU | CCCAAACTACAGATCCCAGC |
| SD | TGGGTAGGGATCACAAGACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17347A-C10 | VGB6 | C57BL/6NTac | |
| 17347A-D9 | VGB6 | C57BL/6NTac | |
| 17347A-E9 | VGB6 | C57BL/6NTac | |
| 17347A-H1 | VGB6 | C57BL/6NTac | |
| 17347A-H9 | VGB6 | C57BL/6NTac | |
