| Gene | Dpm2 |
| Chromosome | 2 |
| Deletion Start | 32,573,022 |
| Deletion End | 32,570,913 |
| Deletion Size | 2,110 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,571,211 | 32,571,230 | CGGGACAGACCAAGCAGTAG |
| TUP | 32,571,232 | 32,571,257 | ATTTGGCCTTGTCGCCGTTAGCTTGA |
| TUR | 32,571,259 | 32,571,283 | CCAAGTGGTGTAGTAGGTGAAGATG |
| TDF | 32,572,608 | 32,572,632 | GTCTCAGCTTCATCTTCATCTCTTC |
| TDP | 32,572,637 | 32,572,659 | TTCCTGCCTCAAGTCACCCTGGC |
| TDR | 32,572,673 | 32,572,692 | ACACAGTTCCCAACCCACAG |
PCR Primers
| Primer | Sequence |
| SU | CTATCCCTTTCCGGGACTTC |
| SD | GTCAGACTCGGAAGAGCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17350A-A9 | VGB6 | C57BL/6NTac | |
| 17350A-B5 | VGB6 | C57BL/6NTac | |
| 17350A-H8 | VGB6 | C57BL/6NTac | |
| 17350A-B11 | VGB6 | C57BL/6NTac | |
| 17350A-H4 | VGB6 | C57BL/6NTac | |
| 17350A-E12 | VGB6 | C57BL/6NTac | |
