| Gene | Edf1 |
| Chromosome | 2 |
| Deletion Start | 25,561,994 |
| Deletion End | 25,557,955 |
| Deletion Size | 4,040 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,558,586 | 25,558,603 | TGAGCCCTGGATGCTGTG |
| TUP | 25,558,606 | 25,558,632 | TCCCTAAGTGGTACTGACCAGAGCTGG |
| TUR | 25,558,642 | 25,558,664 | TGTGGACTTGGCTTCAACTATGG |
| TDF | 25,561,599 | 25,561,618 | CACATCAGCCTGCCTACCTC |
| TDP | 25,561,619 | 25,561,644 | AGTCTCCTGAGCTCTGGGATTGCTGC |
| TDR | 25,561,678 | 25,561,699 | GTGGGTCAAGCATCTCAGAAAG |
PCR Primers
| Primer | Sequence |
| SU | AGCCACAGGGACACGGGA |
| SD | AGAGCACAGGCCCTTAGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17352A-A5 | VGB6 | C57BL/6NTac | |
| 17352A-F7 | VGB6 | C57BL/6NTac | |
| 17352A-E6 | VGB6 | C57BL/6NTac | |
| 17352A-E3 | VGB6 | C57BL/6NTac | |
| 17352A-F6 | VGB6 | C57BL/6NTac | |
| 17352A-C6 | VGB6 | C57BL/6NTac | |
