| Gene | Efha1 |
| Chromosome | 14 |
| Deletion Start | 57,999,236 |
| Deletion End | 57,971,657 |
| Deletion Size | 27,580 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,998,594 | 57,998,612 | GCCTACGCGGCAAAACAAG |
| TUP | 57,998,565 | 57,998,590 | TCCTAAACCTGAGCTGTCAGGATGCA |
| TUR | 57,998,520 | 57,998,542 | GGTTTTACAAGACCGGGTTCATC |
| TDF | 57,972,560 | 57,972,583 | TGCAAACAACACAATCAACCATCC |
| TDP | 57,972,524 | 57,972,550 | AAATGGCACCTCCTCAAGAGCTGTCGC |
| TDR | 57,972,491 | 57,972,512 | CCGTCAACCTCCAACTCAGAAC |
PCR Primers
| Primer | Sequence |
| SU | TTCCACCCAGAATCTATTACCC |
| SD | GATGCCCAATACAAGGAATAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17353A-F12 | VGB6 | C57BL/6NTac | |
| 17353A-C9 | VGB6 | C57BL/6NTac | |
| 17353A-G8 | VGB6 | C57BL/6NTac | |
| 17353A-E9 | VGB6 | C57BL/6NTac | |
