| Gene | Ehd1 |
| Chromosome | 19 |
| Deletion Start | 6,298,643 |
| Deletion End | 6,276,978 |
| Deletion Size | 21,666 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,277,138 | 6,277,157 | TCGACAACAAGCCGATGGTG |
| TUP | 6,277,159 | 6,277,180 | TCCTGGTCGGCCAGTACAGCAC |
| TUR | 6,277,193 | 6,277,212 | CGATCAGGTGGCGGATGAAG |
| TDF | 6,298,469 | 6,298,487 | GGATGGCCTGCTGGATGAC |
| TDP | 6,298,489 | 6,298,511 | AGGAGTTTGCCCTGGCCAACCAC |
| TDR | 6,298,551 | 6,298,573 | GGAATGAGATGTGGAGGAAGGTC |
PCR Primers
| Primer | Sequence |
| SU | CTCGACGAGGTTCGCGCGA |
| SD | AGGTGCCTGGCTTGGATAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17354A-D10 | VGB6 | C57BL/6NTac | |
| 17354A-H1 | VGB6 | C57BL/6NTac | |
| 17354A-H9 | VGB6 | C57BL/6NTac | |
