| Gene | Evc |
| Chromosome | 5 |
| Deletion Start | 37,336,790 |
| Deletion End | 37,300,291 |
| Deletion Size | 36,500 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,336,281 | 37,336,301 | CAAATGCCCACTCCCTCTTTC |
| TUP | 37,336,257 | 37,336,280 | TTGCTTTGGAGCTGCCAGACAACA |
| TUR | 37,336,185 | 37,336,205 | GCTCACCGTTGTGCATTCAAG |
| TDF | 37,301,120 | 37,301,141 | GGGTGAAGCAGAAACAAGGAAC |
| TDP | 37,301,095 | 37,301,115 | TGCACCCAGTTCCATCCCAGC |
| TDR | 37,301,032 | 37,301,051 | CACTGCGAAGCAAGCTGAAC |
PCR Primers
| Primer | Sequence |
| SU | AATCGGATTGGACAGGCTAC |
| SD | GGGACAGGAGATGACTGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 117 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17356A-E11 | VGB6 | C57BL/6NTac | |
| 17356A-G9 | VGB6 | C57BL/6NTac | |
