| Gene | F630043A04Rik |
| Chromosome | 14 |
| Deletion Start | 57,826,109 |
| Deletion End | 57,807,973 |
| Deletion Size | 18,137 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,825,936 | 57,825,956 | GGGCGTAGCTGATCTGTGATG |
| TUP | 57,825,906 | 57,825,930 | TGTGCGGACAAATTCTCCTTGTGGC |
| TUR | 57,825,854 | 57,825,873 | ACAGTTGCCACTGGATGTTG |
| TDF | 57,808,702 | 57,808,726 | GAGGGCTTGTTTATGAGTGGCTATG |
| TDP | 57,808,678 | 57,808,701 | CCACTGGCGTCAGGGCTTCACTCT |
| TDR | 57,808,656 | 57,808,677 | GGGAACCGAGACACAGTGAATG |
PCR Primers
| Primer | Sequence |
| SU | CTGATGCTAGAGTTCCGCTC |
| SD | CAGAACAGAAGGCAACATCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17357A-A6 | VGB6 | C57BL/6NTac | |
| 17357A-A8 | VGB6 | C57BL/6NTac | |
| 17357A-A10 | VGB6 | C57BL/6NTac | |
| 17357A-G3 | VGB6 | C57BL/6NTac | |
| 17357A-F12 | VGB6 | C57BL/6NTac | |
| 17357A-C3 | VGB6 | C57BL/6NTac | |
