| Gene | Fabp1 |
| Chromosome | 6 |
| Deletion Start | 71,204,954 |
| Deletion End | 71,199,930 |
| Deletion Size | 5,025 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,199,958 | 71,199,980 | GCCAGGAGAACTTTGAGCCATTC |
| TUP | 71,199,992 | 71,200,019 | AGGTGAGTGGTGATTATGACAGCAACGA |
| TUR | 71,200,042 | 71,200,063 | TCAACACCAGACCTGGACTATC |
| TDF | 71,204,148 | 71,204,170 | CACAGCCCAGGTGCCTAATAAAC |
| TDP | 71,204,172 | 71,204,195 | CCTCTGCCCACTTGGCATTGGTGT |
| TDR | 71,204,235 | 71,204,254 | GAGTGCATACCCAGCGTGTC |
PCR Primers
| Primer | Sequence |
| SU | TTACAGAGAGCTTTGCCCTTG |
| SD | GGATCCCTGTCCTTATGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17358A-B12 | VGB6 | C57BL/6NTac | |
| 17358A-C11 | VGB6 | C57BL/6NTac | |
| 17358A-D11 | VGB6 | C57BL/6NTac | |
| 17358A-E2 | VGB6 | C57BL/6NTac | |
| 17358A-H7 | VGB6 | C57BL/6NTac | |
| 17358A-E5 | VGB6 | C57BL/6NTac | |
