| Gene | Fam110b |
| Chromosome | 4 |
| Deletion Start | 5,799,736 |
| Deletion End | 5,798,587 |
| Deletion Size | 1,150 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,798,886 | 5,798,903 | CGGCAACCATGCCAAGAC |
| TUP | 5,798,908 | 5,798,930 | AGCGGTGTGCAACGTGAGACCCT |
| TUR | 5,798,949 | 5,798,971 | CCTCTGAGCTGTTGATGATGTTC |
| TDF | 5,799,359 | 5,799,382 | TGAGTGACAGGTATTTCCGAGTAG |
| TDP | 5,799,390 | 5,799,417 | TGTGGAGAGGTTCTTCAACTACTGCGGA |
| TDR | 5,799,442 | 5,799,462 | TGCAAAGTTCTCCATGCCAAG |
PCR Primers
| Primer | Sequence |
| SU | TGCAGTTGAGCACAAAGACC |
| SD | TCCCAGTGTTATGGTCTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17359A-H12 | VGB6 | C57BL/6NTac | |
| 17359A-F2 | VGB6 | C57BL/6NTac | |
