| Gene | Fam165b |
| Chromosome | 16 |
| Deletion Start | 92,310,992 |
| Deletion End | 92,305,389 |
| Deletion Size | 5,604 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,305,890 | 92,305,911 | TCCTGGCTCTCATCATCTCTTC |
| TUP | 92,305,912 | 92,305,938 | CCACTCCTGTGAACGTCCATTACTCCC |
| TUR | 92,305,947 | 92,305,967 | CCAAGGCATGGGTGTGAGTAG |
| TDF | 92,310,676 | 92,310,694 | CCATTGTGGTCCAGGAGTC |
| TDP | 92,310,696 | 92,310,722 | AGCCTCAGCTTGAAACATTGTAGGTGC |
| TDR | 92,310,746 | 92,310,767 | CAGTGATGTGGAGTCTCGGTTC |
PCR Primers
| Primer | Sequence |
| SU | CTACTTGCTCTCTGTTCACAG |
| SD | AAGGGATCATTTGCCAACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17360A-A10 | VGB6 | C57BL/6NTac | |
| 17360A-H6 | VGB6 | C57BL/6NTac | |
| 17360A-C2 | VGB6 | C57BL/6NTac | |
| 17360A-E1 | VGB6 | C57BL/6NTac | |
| 17360A-G12 | VGB6 | C57BL/6NTac | |
| 17360A-F9 | VGB6 | C57BL/6NTac | |
