| Gene | Fhod1 |
| Chromosome | 8 |
| Deletion Start | 105,347,850 |
| Deletion End | 105,336,387 |
| Deletion Size | 11,464 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,347,410 | 105,347,430 | CCTGAAGAGGTGCTTGGAGTC |
| TUP | 105,347,390 | 105,347,409 | CCCTCGCTGCCTCTTTGCCA |
| TUR | 105,347,371 | 105,347,388 | ACAACCACAGCAGCAACC |
| TDF | 105,337,209 | 105,337,227 | TGTGCGGCAGGTACTCTTC |
| TDP | 105,337,185 | 105,337,207 | CTGGGCCAACTTGGTGTCCATCC |
| TDR | 105,337,156 | 105,337,177 | CTCTGCATCAGCTCCATTCTTC |
PCR Primers
| Primer | Sequence |
| SU | TAACGGGATCACCAGCTCTC |
| SD | AGGTGAGGTGGAACACTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 60 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17362A-B4 | VGB6 | C57BL/6NTac | |
| 17362A-G3 | VGB6 | C57BL/6NTac | |
| 17362A-D12 | VGB6 | C57BL/6NTac | |
| 17362A-E4 | VGB6 | C57BL/6NTac | |
| 17362A-E5 | VGB6 | C57BL/6NTac | |
