| Gene | Fpgs |
| Chromosome | 2 |
| Deletion Start | 32,694,123 |
| Deletion End | 32,682,963 |
| Deletion Size | 11,161 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,693,422 | 32,693,441 | GCTTCCAGCCAAGCCTTGAG |
| TUP | 32,693,398 | 32,693,420 | ATAAGAGGGCAGGCGACCCAGCT |
| TUR | 32,693,367 | 32,693,390 | ACCTGTGCTTAGGCAGTTCCTTTC |
| TDF | 32,683,674 | 32,683,692 | CAGGCTTCCTGGGAAACAC |
| TDP | 32,683,652 | 32,683,673 | TCATGCCCACACGTGAGGGACC |
| TDR | 32,683,622 | 32,683,643 | GCACGCTCACCTTTACAAACTG |
PCR Primers
| Primer | Sequence |
| SU | GGACTGAGAACTGCTGGCTC |
| SD | AGTTCCAGCAACACAGTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17363A-A7 | VGB6 | C57BL/6NTac | |
| 17363A-A11 | VGB6 | C57BL/6NTac | |
| 17363A-F11 | VGB6 | C57BL/6NTac | |
| 17363A-F1 | VGB6 | C57BL/6NTac | |
| 17363A-D7 | VGB6 | C57BL/6NTac | |
