| Gene | Frmd8 |
| Chromosome | 19 |
| Deletion Start | 5,874,791 |
| Deletion End | 5,852,581 |
| Deletion Size | 22,211 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,874,727 | 5,874,745 | CCCACCGAAGTAGCGTCTC |
| TUP | 5,874,695 | 5,874,723 | AGTGGGTGCCCGAGGTAGGTCTCTACTTT |
| TUR | 5,874,655 | 5,874,674 | CCCAGGCTGGAACCAAAGAG |
| TDF | 5,852,997 | 5,853,017 | GCTTCCCATCCTCAGCTATCC |
| TDP | 5,852,972 | 5,852,995 | TGTCTTAGTGGTCGTCACCCAGCA |
| TDR | 5,852,947 | 5,852,967 | AAGCTCCAGAAGACTCCAGAC |
PCR Primers
| Primer | Sequence |
| SU | ATTCTGGAGTGAGGCAGTGG |
| SD | ACCGAAGGCAAACACTGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17364A-A12 | VGB6 | C57BL/6NTac | |
| 17364A-B7 | VGB6 | C57BL/6NTac | |
| 17364A-C3 | VGB6 | C57BL/6NTac | |
| 17364A-C6 | VGB6 | C57BL/6NTac | |
| 17364A-H6 | VGB6 | C57BL/6NTac | |
