| Gene | Gad2 |
| Chromosome | 2 |
| Deletion Start | 22,630,056 |
| Deletion End | 22,622,666 |
| Deletion Size | 7,391 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 22,623,096 | 22,623,113 | GCAAGCGCGGTGAGAAAC |
| TUP | 22,623,115 | 22,623,141 | AACAGCCTCGGGTTTAGGAAAGGCAGC |
| TUR | 22,623,184 | 22,623,203 | CCGCCCTTTGCACTTTGTTC |
| TDF | 22,629,387 | 22,629,411 | TCAGGGCATCCCCGATATTTTAATC |
| TDP | 22,629,420 | 22,629,449 | ACTGGATTGGATATGGTTGGATTAGCAGCA |
| TDR | 22,629,452 | 22,629,473 | GTTTGCTGTTGATGTCAACCAG |
PCR Primers
| Primer | Sequence |
| SU | CCGTCACTCTCTCATCTCCC |
| SD | TTCCGAGAAATGCAGCAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17366A-G1 | VGB6 | C57BL/6NTac | |
| 17366A-D1 | VGB6 | C57BL/6NTac | |
| 17366A-D5 | VGB6 | C57BL/6NTac | |
| 17366A-F6 | VGB6 | C57BL/6NTac | |
| 17366A-D12 | VGB6 | C57BL/6NTac | |
| 17366A-A11 | VGB6 | C57BL/6NTac | |
