| Gene | Gfpt2 |
| Chromosome | 11 |
| Deletion Start | 49,824,034 |
| Deletion End | 49,794,240 |
| Deletion Size | 29,795 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 49,794,731 | 49,794,751 | CCTAGAAGGGTGGGAACTGTG |
| TUP | 49,794,756 | 49,794,781 | AGCTTTGCTGGAACGAGCTCTTGGCG |
| TUR | 49,794,817 | 49,794,835 | GCTCCCGCTCCTAGCTTTG |
| TDF | 49,823,924 | 49,823,942 | TGCCTCGTGTCTTGCAGTG |
| TDP | 49,823,944 | 49,823,967 | TCCTGGGTGGCTTGAAGGACCATT |
| TDR | 49,823,969 | 49,823,989 | CGGCATCGTCGGATCTCTTTC |
PCR Primers
| Primer | Sequence |
| SU | TTACCACCGATTGGGCTTC |
| SD | CTAGGCGTCTAAGGGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17372A-C8 | VGB6 | C57BL/6NTac | |
| 17372A-B11 | VGB6 | C57BL/6NTac | |
