| Gene | Glis2 |
| Chromosome | 16 |
| Deletion Start | 4,614,176 |
| Deletion End | 4,608,650 |
| Deletion Size | 5,527 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,609,276 | 4,609,296 | CGCCTTACGCCATTGACATTC |
| TUP | 4,609,297 | 4,609,324 | CCCAATGAACACACACAGGACAGACACA |
| TUR | 4,609,342 | 4,609,361 | GAGGTTAGGCCCTGCTACTG |
| TDF | 4,613,320 | 4,613,344 | GGAGAGGCAAGAGAATAGTGGAAAG |
| TDP | 4,613,346 | 4,613,370 | TCTGTCAGTTGCCAGCACTCAGTGC |
| TDR | 4,613,408 | 4,613,427 | GCTTGTTGCAGCCCTCATAG |
PCR Primers
| Primer | Sequence |
| SU | TGCCAGACTGTGAGTTCTGC |
| SD | GAGCAAGAGGCAGGACTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17373A-A1 | VGB6 | C57BL/6NTac | |
| 17373A-A11 | VGB6 | C57BL/6NTac | |
| 17373A-E4 | VGB6 | C57BL/6NTac | |
| 17373A-D11 | VGB6 | C57BL/6NTac | |
