| Gene | Gna15 |
| Chromosome | 10 |
| Deletion Start | 81,523,951 |
| Deletion End | 81,502,850 |
| Deletion Size | 21,102 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,523,170 | 81,523,189 | AGGCACGGGAGGTACGATTG |
| TUP | 81,523,147 | 81,523,169 | TGTCACCAAGCATTGCGGAGACA |
| TUR | 81,523,121 | 81,523,142 | CCCTGATCCCATGATGTTCCTG |
| TDF | 81,503,544 | 81,503,564 | GCAGCAAGCACTCTTATCCTC |
| TDP | 81,503,520 | 81,503,543 | TGAGCCATTGCCTTTGTCCTGGAA |
| TDR | 81,503,495 | 81,503,515 | GGAACTCTGTGGGCTCGAATC |
PCR Primers
| Primer | Sequence |
| SU | TTCTGAAATCAGCCCTGAGC |
| SD | CAGGAAGATGTGAACAGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17375A-A7 | VGB6 | C57BL/6NTac | |
| 17375A-H11 | VGB6 | C57BL/6NTac | |
| 17375A-G8 | VGB6 | C57BL/6NTac | |
| 17375A-G2 | VGB6 | C57BL/6NTac | |
| 17375A-F9 | VGB6 | C57BL/6NTac | |
| 17375A-E12 | VGB6 | C57BL/6NTac | |
