| Gene | Gnat2 |
| Chromosome | 3 |
| Deletion Start | 108,100,898 |
| Deletion End | 108,093,252 |
| Deletion Size | 7,647 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,093,327 | 108,093,346 | GCTGACAAGGAAGCCAAGAC |
| TUP | 108,093,349 | 108,093,370 | TCAAGCTGCTGTTGCTCGGTGA |
| TUR | 108,093,400 | 108,093,422 | CTGAACCAGAGGGAGAGGGATAC |
| TDF | 108,100,254 | 108,100,276 | TCTGGGAAAGAGCAGACAGATAC |
| TDP | 108,100,278 | 108,100,301 | CCAGAGCCACTGACCGTTCCAAGG |
| TDR | 108,100,303 | 108,100,324 | GGGTCTGACTTTCTGGTAGGTG |
PCR Primers
| Primer | Sequence |
| SU | CAGAAGCCAGATCATTTCCC |
| SD | ATGGTAGTCCATCAGGCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17376A-B1 | VGB6 | C57BL/6NTac | |
| 17376A-C6 | VGB6 | C57BL/6NTac | |
| 17376A-H7 | VGB6 | C57BL/6NTac | |
| 17376A-C12 | VGB6 | C57BL/6NTac | |
| 17376A-D8 | VGB6 | C57BL/6NTac | |
| 17376A-C11 | VGB6 | C57BL/6NTac | |
