| Gene | Gnaz |
| Chromosome | 10 |
| Deletion Start | 75,015,307 |
| Deletion End | 74,991,421 |
| Deletion Size | 23,887 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 74,992,069 | 74,992,086 | GGCGTCACAGCCATCATC |
| TUP | 74,992,087 | 74,992,112 | TTCTGTGTGGAGCTCAGTGGCTATGA |
| TUR | 74,992,122 | 74,992,143 | CACCGTCTGGTTGTCCTCATAG |
| TDF | 75,015,045 | 75,015,065 | GCGGTCTACATCCAACGTCAG |
| TDP | 75,015,067 | 75,015,090 | TCGAGGACCTCAACCGCAACAAGG |
| TDR | 75,015,113 | 75,015,131 | GTCGGTGGCACAAGTGAAG |
PCR Primers
| Primer | Sequence |
| SU | ATGACTACGGCAAATCAGGC |
| SD | TCCAGAGCTCAGTTGGTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17377A-A1 | VGB6 | C57BL/6NTac | |
| 17377A-G2 | VGB6 | C57BL/6NTac | |
| 17377A-D2 | VGB6 | C57BL/6NTac | |
| 17377A-D3 | VGB6 | C57BL/6NTac | |
| 17377A-B1 | VGB6 | C57BL/6NTac | |
| 17377A-G6 | VGB6 | C57BL/6NTac | |
