| Gene | Gng7 |
| Chromosome | 10 |
| Deletion Start | 80,952,911 |
| Deletion End | 80,951,531 |
| Deletion Size | 1,381 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,952,653 | 80,952,670 | TCAGGCCGGTTCAGTGTC |
| TUP | 80,952,625 | 80,952,651 | TCCTCTGGTTACCACAGATGCAAGCAG |
| TUR | 80,952,596 | 80,952,614 | TCTGCGCCATTCCTCTGTG |
| TDF | 80,951,766 | 80,951,787 | GTCCCATCTGACCTTTCCTTTC |
| TDP | 80,951,740 | 80,951,764 | CCTCCTTGTGCTACACAGGTCTCCA |
| TDR | 80,951,697 | 80,951,717 | CGGGCATGTTGCTCACAGTAG |
PCR Primers
| Primer | Sequence |
| SU | TCACTGGTTTCACTGCCATC |
| SD | CAAGGCAACTAAGGGGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17378A-B8 | VGB6 | C57BL/6NTac | |
| 17378A-C3 | VGB6 | C57BL/6NTac | |
| 17378A-F10 | VGB6 | C57BL/6NTac | |
| 17378A-G5 | VGB6 | C57BL/6NTac | |
| 17378A-E3 | VGB6 | C57BL/6NTac | |
| 17378A-G2 | VGB6 | C57BL/6NTac | |
