| Gene | Gorasp2 |
| Chromosome | 2 |
| Deletion Start | 70,690,991 |
| Deletion End | 70,661,701 |
| Deletion Size | 29,291 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,662,434 | 70,662,456 | TCTCTGAGAGTGTGCTTGCTTAC |
| TUP | 70,662,463 | 70,662,485 | TTTCTGCCCTGGGTCGCATGTTC |
| TUR | 70,662,515 | 70,662,537 | CACAGTCCTATCGTTCAGGTATC |
| TDF | 70,690,536 | 70,690,556 | CAAAGGTGGCAGTGAGCAAAG |
| TDP | 70,690,557 | 70,690,578 | AGCTCTGCTTCGGCTGCAGGCT |
| TDR | 70,690,590 | 70,690,610 | ACGGCCATCAAGCTCCTCTAC |
PCR Primers
| Primer | Sequence |
| SU | CTGCGAAGGTAGAAACGC |
| SD | TCCAAAGATGAACCTCCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17379A-C2 | VGB6 | C57BL/6NTac | |
| 17379A-C8 | VGB6 | C57BL/6NTac | |
| 17379A-E10 | VGB6 | C57BL/6NTac | |
| 17379A-C11 | VGB6 | C57BL/6NTac | |
| 17379A-A1 | VGB6 | C57BL/6NTac | |
