| Gene | Grpel1 |
| Chromosome | 5 |
| Deletion Start | 36,471,477 |
| Deletion End | 36,465,197 |
| Deletion Size | 6,281 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,465,317 | 36,465,334 | TTAGCGGCAGGACTGGTC |
| TUP | 36,465,343 | 36,465,364 | CGGTGCCCGTGCACCCTGAAGT |
| TUR | 36,465,365 | 36,465,385 | GGACTCGATGGTGAGGATGTG |
| TDF | 36,471,052 | 36,471,069 | GCAGGCATCCAGGGTTTC |
| TDP | 36,471,072 | 36,471,097 | CAAGGACTTGCTGGAGGTTGCAGACA |
| TDR | 36,471,117 | 36,471,138 | GATCTCCTCCTTTGGAACACTC |
PCR Primers
| Primer | Sequence |
| SU | TAGCAGGAGCCTAAAGCGAG |
| SD | AAGTGTTCCTGCTCTGGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17380A-A2 | VGB6 | C57BL/6NTac | |
| 17380A-G1 | VGB6 | C57BL/6NTac | |
| 17380A-G3 | VGB6 | C57BL/6NTac | |
| 17380A-G5 | VGB6 | C57BL/6NTac | |
