| Gene | Gsn |
| Chromosome | 2 |
| Deletion Start | 35,307,703 |
| Deletion End | 35,282,467 |
| Deletion Size | 25,237 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,283,135 | 35,283,154 | GTCTGAGGTGACCTGCTGAC |
| TUP | 35,283,156 | 35,283,177 | TCCCTCCTTGGGCGCAAACCTA |
| TUR | 35,283,198 | 35,283,218 | GCTGCCAGACATGCTCTGTAG |
| TDF | 35,307,600 | 35,307,620 | CGGCTGGGATGACAACTACTG |
| TDP | 35,307,626 | 35,307,647 | TGGATCCTTTGGACCGGGCCTT |
| TDR | 35,307,667 | 35,307,690 | GACATTGATGGCTTTGGTCCTTAC |
PCR Primers
| Primer | Sequence |
| SU | TACTTGAGTTGGAAACCGCC |
| SD | CTTCTCAAGGACCGAAATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17381A-H6 | VGB6 | C57BL/6NTac | |
| 17381A-E2 | VGB6 | C57BL/6NTac | |
| 17381A-F11 | VGB6 | C57BL/6NTac | |
