| Gene | Gtf2e1 |
| Chromosome | 16 |
| Deletion Start | 37,536,165 |
| Deletion End | 37,511,319 |
| Deletion Size | 24,847 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,535,314 | 37,535,335 | GCATTGTGCTGTTAGGAGTGTG |
| TUP | 37,535,286 | 37,535,313 | TAACTAGGATGCGTGATCTGCGTTTGGG |
| TUR | 37,535,253 | 37,535,274 | GGGTCTCACCTAGTCCTTCTTC |
| TDF | 37,511,404 | 37,511,423 | TGGGCCAGCGTATGTTTGAG |
| TDP | 37,511,373 | 37,511,399 | TCTTTGAGTGAGCTTTCCCTGCTCTCC |
| TDR | 37,511,335 | 37,511,354 | GCACCTGATGGGAGTTTCTC |
PCR Primers
| Primer | Sequence |
| SU | ACTGTGAGCACTCCCAGAGC |
| SD | CCATTTACAGACTGGCCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17382A-D6 | VGB6 | C57BL/6NTac | |
| 17382A-D8 | VGB6 | C57BL/6NTac | |
| 17382A-E12 | VGB6 | C57BL/6NTac | |
| 17382A-G6 | VGB6 | C57BL/6NTac | |
| 17382A-H1 | VGB6 | C57BL/6NTac | |
| 17382A-H10 | VGB6 | C57BL/6NTac | |
