| Gene | Haus3 |
| Chromosome | 5 |
| Deletion Start | 34,168,310 |
| Deletion End | 34,153,977 |
| Deletion Size | 14,334 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,168,119 | 34,168,139 | GAAGCCTTCAGCGATCTCCAG |
| TUP | 34,168,089 | 34,168,118 | CGATCAGGCAAGCCTATCCTAGAAGGAACA |
| TUR | 34,168,065 | 34,168,088 | GGTCCTAAGAACTTCATCCAATGC |
| TDF | 34,154,802 | 34,154,824 | GACATGGAACAGATCAGGTACAC |
| TDP | 34,154,767 | 34,154,791 | AAGAGTTTACATTGAGTCATTCAGA |
| TDR | 34,154,695 | 34,154,719 | AAAGCAAAGAAGTTGTTTCCACCAG |
PCR Primers
| Primer | Sequence |
| SU | TAGTGTCCTAAGCAACACCCTG |
| SD | CATAAACTTAGTAGCATTGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 130 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17383A-B5 | VGB6 | C57BL/6NTac | |
| 17383A-D7 | VGB6 | C57BL/6NTac | |
| 17383A-E3 | VGB6 | C57BL/6NTac | |
| 17383A-E5 | VGB6 | C57BL/6NTac | |
| 17383A-G6 | VGB6 | C57BL/6NTac | |
| 17383A-G9 | VGB6 | C57BL/6NTac | |
