Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

17388 Hspa1l Status: ES cell colonies screened / QC positives

Gene	Hspa1l
Chromosome	17
Deletion Start	34,978,996
Deletion End	34,976,990
Deletion Size	2,007
Genome Build	38

MGI ID	MGI:96231
NCBI Gene ID	15482
BAC ID	371P17
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 17

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	34,977,426	34,977,446	CGGTGCCAGCCTATTTCAATG
TUP	34,977,447	34,977,468	ACTCTCAGCGGCAAGCCACCAA
TUR	34,977,474	34,977,493	GAGTCCTGCGATGACACCTG
TDF	34,978,805	34,978,828	CCGATCATCACAAAACTGTACCAG
TDP	34,978,834	34,978,855	ATGCACCGGGCCCACCTGTACG
TDR	34,978,920	34,978,941	ACCCTAGCACCCTGCAATTCTG

PCR Primers

Primer	Sequence
SU	GCATCTTGAAGAGAAAAACTGATC
SD	GATGACAGAAGTTGTTTCCTTTG
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	68 bp	+	+	-
TDF	TDR	137 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
17388A-H11	VGB6	C57BL/6NTac
17388A-F6	VGB6	C57BL/6NTac
17388A-F1	VGB6	C57BL/6NTac
17388A-H8	VGB6	C57BL/6NTac