| Gene | Hspd1 |
| Chromosome | 1 |
| Deletion Start | 55,086,936 |
| Deletion End | 55,078,190 |
| Deletion Size | 8,747 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,086,164 | 55,086,185 | GGTTGCTGGGATTTGAAGGTTG |
| TUP | 55,086,140 | 55,086,162 | TGGGACCTTCGGAAGAGCAGTCA |
| TUR | 55,086,114 | 55,086,134 | AAAGATGGCTCAGCGGGTAAG |
| TDF | 55,078,543 | 55,078,565 | GAACATGGTGGAAAAAGGGATCA |
| TDP | 55,078,515 | 55,078,538 | TCCAACAAAGGTAAGTGATTCAGC |
| TDR | 55,078,482 | 55,078,502 | TGCAGAGAGCAAGACATCCAG |
PCR Primers
| Primer | Sequence |
| SU | GCATTTAGAACCTGCCTTGC |
| SD | CATCAAGACCATGGAAAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17389A-H10 | VGB6 | C57BL/6NTac | |
| 17389A-A8 | VGB6 | C57BL/6NTac | |
| 17389A-A9 | VGB6 | C57BL/6NTac | |
| 17389A-H8 | VGB6 | C57BL/6NTac | |
| 17389A-F9 | VGB6 | C57BL/6NTac | |
| 17389A-E11 | VGB6 | C57BL/6NTac | |
