| Gene | Immt |
| Chromosome | 6 |
| Deletion Start | 71,863,265 |
| Deletion End | 71,831,474 |
| Deletion Size | 31,792 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,831,839 | 71,831,862 | GATAGGTTTCCAATCGTGAAGGTC |
| TUP | 71,831,865 | 71,831,888 | CGCGGGAGAACTACAGTTCCCAGC |
| TUR | 71,831,892 | 71,831,913 | CAACAAGCCCTGAGAAGCAATC |
| TDF | 71,863,150 | 71,863,170 | GCTCAGTCAGAGGCAAAGGTG |
| TDP | 71,863,174 | 71,863,203 | TCTCAGTATCATGAACTTGTGGTCCAAGCA |
| TDR | 71,863,232 | 71,863,255 | CAGGAGTGATGTCTGGAGTGATAC |
PCR Primers
| Primer | Sequence |
| SU | GGCCAGTTCACCTAGTAACCC |
| SD | GAGTAGCAAATCAGAATCAAATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17390A-E2 | VGB6 | C57BL/6NTac | |
| 17390A-F10 | VGB6 | C57BL/6NTac | |
| 17390A-G7 | VGB6 | C57BL/6NTac | |
| 17390A-H11 | VGB6 | C57BL/6NTac | |
