| Gene | Incenp |
| Chromosome | 19 |
| Deletion Start | 9,895,457 |
| Deletion End | 9,872,670 |
| Deletion Size | 22,788 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 9,894,592 | 9,894,610 | GGGAGAGGCACCAGAGTTG |
| TUP | 9,894,567 | 9,894,591 | TGGCCCAGGTTGCTGGTTCTTACCT |
| TUR | 9,894,519 | 9,894,536 | TGCAGTCACCACCATTCC |
| TDF | 9,872,997 | 9,873,017 | AGCAGGAGGTTGCTAGAGGAG |
| TDP | 9,872,966 | 9,872,991 | AGGATAGGAGCAAGGCATGCTGTCAT |
| TDR | 9,872,941 | 9,872,959 | CCCACAGAGCCCAGTCATC |
PCR Primers
| Primer | Sequence |
| SU | AGCCCACAAGAAGGTAAACG |
| SD | GGCACAAGACATACTGCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17391A-A9 | VGB6 | C57BL/6NTac | |
| 17391A-G5 | VGB6 | C57BL/6NTac | |
| 17391A-A6 | VGB6 | C57BL/6NTac | |
