| Gene | Iqce |
| Chromosome | 5 |
| Deletion Start | 140,702,214 |
| Deletion End | 140,665,311 |
| Deletion Size | 36,904 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 140,701,685 | 140,701,708 | GAGCAGCTTGGTACAGGTTTAGAG |
| TUP | 140,701,655 | 140,701,684 | CCAGTAGGAGCATTAAATGTAAAGGCCGCA |
| TUR | 140,701,626 | 140,701,646 | GCTTTCCGGTGTGCTACTTTC |
| TDF | 140,665,417 | 140,665,437 | TGCTCGGATGACTCAGATGAC |
| TDP | 140,665,386 | 140,665,409 | TTTCGCCATTTCTGCCCAGGAAGA |
| TDR | 140,665,337 | 140,665,356 | CACCTCACCTGGAGGCAATG |
PCR Primers
| Primer | Sequence |
| SU | GCCCACAAGACCACAAGA |
| SD | ATGGCCATAGGTTCTTGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17392A-D4 | VGB6 | C57BL/6NTac | |
| 17392A-E8 | VGB6 | C57BL/6NTac | |
