| Gene | Irak2 |
| Chromosome | 6 |
| Deletion Start | 113,672,936 |
| Deletion End | 113,638,551 |
| Deletion Size | 34,386 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,639,287 | 113,639,307 | GTAGCCCTGGTACTTGCTTTG |
| TUP | 113,639,309 | 113,639,333 | AGATCAGGCTGGCCTGGAACTAACA |
| TUR | 113,639,337 | 113,639,357 | CAGCAGGAGAGGCAGGTAGAG |
| TDF | 113,672,781 | 113,672,798 | TTGGGTTTGCCCGGAGAC |
| TDP | 113,672,804 | 113,672,830 | TTTCTGGAGTGAAGCAGATGTCGTCCA |
| TDR | 113,672,850 | 113,672,868 | CGCTGATTCGGTGGCTTTG |
PCR Primers
| Primer | Sequence |
| SU | TCAGCACAAGGTTTGATTCG |
| SD | TCTCCGTGACAGCCTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17393A-A11 | VGB6 | C57BL/6NTac | |
| 17393A-C6 | VGB6 | C57BL/6NTac | |
| 17393A-D1 | VGB6 | C57BL/6NTac | |
| 17393A-F9 | VGB6 | C57BL/6NTac | |
| 17393A-E1 | VGB6 | C57BL/6NTac | |
| 17393A-F11 | VGB6 | C57BL/6NTac | |
