| Gene | Magix |
| Chromosome | X |
| Deletion Start | 7,680,995 |
| Deletion End | 7,674,900 |
| Deletion Size | 6,096 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,680,288 | 7,680,309 | TGTTGAGTGCTCAGAGGTGTTC |
| TUP | 7,680,256 | 7,680,284 | TCTATTGCTGTCAAATGGCTACCTTCGGG |
| TUR | 7,680,227 | 7,680,244 | CATTGCCCGCTGCCTTAC |
| TDF | 7,675,213 | 7,675,237 | TGTCTATTCCACCACCCAATCTAAC |
| TDP | 7,675,183 | 7,675,209 | TAGTCTTGCTATCTCCAGATCGCAGCC |
| TDR | 7,675,153 | 7,675,171 | GAGACTCCACCCTGCTTCC |
PCR Primers
| Primer | Sequence |
| SU | GTCTCCAGTTAAGAGAAGCAG |
| SD | CGAAGGGACTTCATCGAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17399A-B8 | VGB6 | C57BL/6NTac | |
| 17399A-C7 | VGB6 | C57BL/6NTac | |
| 17399A-D2 | VGB6 | C57BL/6NTac | |
| 17399A-D8 | VGB6 | C57BL/6NTac | |
| 17399A-E5 | VGB6 | C57BL/6NTac | |
| 17399A-F12 | VGB6 | C57BL/6NTac | |
