Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

17400 Man1b1 Status: ES cell colonies screened / QC positives

Gene	Man1b1
Chromosome	2
Deletion Start	25,350,649
Deletion End	25,332,863
Deletion Size	17,787
Genome Build	38

MGI ID	MGI:2684954
NCBI Gene ID	227619
BAC ID	446L5
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	25,333,036	25,333,055	GTGTCTCTTCCAGCGTCCAG
TUP	25,333,059	25,333,083	TTTCTGTGCTGCGTCTGGTTCTCGC
TUR	25,333,090	25,333,109	GAAGCAACCACACCGAAACC
TDF	25,350,007	25,350,028	AGAGCTGGATGTGGTCTAAGTG
TDP	25,350,029	25,350,054	TGACACCTAGCTCTTTGCTCCAGACC
TDR	25,350,068	25,350,086	GGCAGGCACAGGCAAGAAG

PCR Primers

Primer	Sequence
SU	AAGGACGCGATGAAATAACG
SD	GAGCCACAAAGACCCTCAAG
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	74 bp	+	+	-
TDF	TDR	80 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
17400A-D5	VGB6	C57BL/6NTac
17400A-F3	VGB6	C57BL/6NTac
17400A-G8	VGB6	C57BL/6NTac
17400A-H4	VGB6	C57BL/6NTac