| Gene | Map2k2 |
| Chromosome | 10 |
| Deletion Start | 81,123,795 |
| Deletion End | 81,106,150 |
| Deletion Size | 17,646 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,107,036 | 81,107,059 | GGTGTGGTATGGTTAGAGGAACAG |
| TUP | 81,107,073 | 81,107,099 | TAGCCTAGCCTAGCGCTTAAGACTGGG |
| TUR | 81,107,118 | 81,107,139 | TCAGACAAGTCATGCCTTCAAC |
| TDF | 81,123,441 | 81,123,461 | TGTGTCCTTTGCACCTAAGTC |
| TDP | 81,123,463 | 81,123,486 | CCTCTGCCAAGGAGTCAGCAGTGG |
| TDR | 81,123,557 | 81,123,575 | CTCAGGTGGCCTGGCTATC |
PCR Primers
| Primer | Sequence |
| SU | GAGCCACCTCGCGCATGC |
| SD | GTTCCATGACCCAGATCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 135 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17401A-C5 | VGB6 | C57BL/6NTac | |
| 17401A-E5 | VGB6 | C57BL/6NTac | |
| 17401A-D10 | VGB6 | C57BL/6NTac | |
