| Gene | Mlxipl |
| Chromosome | 5 |
| Deletion Start | 135,137,373 |
| Deletion End | 135,106,926 |
| Deletion Size | 30,448 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 135,107,557 | 135,107,577 | GCCTGGACTGTTTCGTTCATC |
| TUP | 135,107,578 | 135,107,601 | CTCAGCAGTCCCTGCAAAGTCCCA |
| TUR | 135,107,619 | 135,107,637 | GGCGACCACGGGATAACTG |
| TDF | 135,137,274 | 135,137,294 | TCCACAAGCATCCTGACTGAC |
| TDP | 135,137,295 | 135,137,315 | CCCAGCCTTGTGCCTGAGCAA |
| TDR | 135,137,326 | 135,137,345 | CAGGGTGCCCTCTGTGACTG |
PCR Primers
| Primer | Sequence |
| SU | GCCAGACTTTCTGAAACCTG |
| SD | GAAGCCACCCTATAGCTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17405A-B5 | VGB6 | C57BL/6NTac | |
| 17405A-D3 | VGB6 | C57BL/6NTac | |
| 17405A-G1 | VGB6 | C57BL/6NTac | |
| 17405A-F1 | VGB6 | C57BL/6NTac | |
| 17405A-F2 | VGB6 | C57BL/6NTac | |
| 17405A-D11 | VGB6 | C57BL/6NTac | |
