| Gene | Mmgt1 |
| Chromosome | X |
| Deletion Start | 56,597,860 |
| Deletion End | 56,589,129 |
| Deletion Size | 8,732 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,597,479 | 56,597,503 | TAAGACAAAGTGTGGAATTGAGAAC |
| TUP | 56,597,448 | 56,597,473 | ATGCATGTGGCATCGTGACAGCTAGC |
| TUR | 56,597,414 | 56,597,431 | TGCCTGGACAGCAGAGTG |
| TDF | 56,589,243 | 56,589,265 | TGTTTAACCATCGTGGTCGAGTG |
| TDP | 56,589,219 | 56,589,241 | TGTTCCGGCCTTCAGATGCAACA |
| TDR | 56,589,149 | 56,589,169 | GGCGCAGTGAGTCAAACTTTC |
PCR Primers
| Primer | Sequence |
| SU | GTATTTGCGGACGCTCTACC |
| SD | CCATTTGATTCTTGTGCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 117 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17406A-B3 | VGB6 | C57BL/6NTac | |
| 17406C-A11 | VGB6 | C57BL/6NTac | |
| 17406C-B11 | VGB6 | C57BL/6NTac | |
