| Gene | Mob4 |
| Chromosome | 1 |
| Deletion Start | 55,152,815 |
| Deletion End | 55,131,303 |
| Deletion Size | 21,513 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,131,769 | 55,131,787 | GGTCGGCTCCATCCAGTTG |
| TUP | 55,131,795 | 55,131,823 | CGGATGCAGGTGGCACTAGTTTGCGTTAT |
| TUR | 55,131,837 | 55,131,857 | GAAGGGAGTGGTGGCAAAGAC |
| TDF | 55,152,726 | 55,152,750 | CTGATGTCGAAGGATAACCTGATTG |
| TDP | 55,152,758 | 55,152,782 | TTTAGAAGAGGAAGTTCAGAACTCA |
| TDR | 55,152,783 | 55,152,803 | TGCTTCACTTTCCCCAGAAAC |
PCR Primers
| Primer | Sequence |
| SU | CAGGCTTGGGTCACCTGC |
| SD | GGGTCCAATGATTGTTTAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17407A-A6 | VGB6 | C57BL/6NTac | |
| 17407A-C3 | VGB6 | C57BL/6NTac | |
| 17407A-H5 | VGB6 | C57BL/6NTac | |
| 17407A-E2 | VGB6 | C57BL/6NTac | |
| 17407A-D3 | VGB6 | C57BL/6NTac | |
